Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck]
Identifieur interne : 003015 ( Main/Exploration ); précédent : 003014; suivant : 003016Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck]
Auteurs : A. Pedrosa [Autriche] ; D. Schweizer [Autriche] ; M. Guerra [Autriche]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 2000.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Agrume.
English descriptors
- KwdEn :
Abstract
Citrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the extension of these heteromorphisms, ten cultivars of C. sinenesis were analysed with CMA/DAP1 staining and, in some of them, the 18S-5.8S-25S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed exactly the same CMA+/DAPI- banding pattern for all cultivars. In situ hybridization revealed three 18S-5.8S-25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromosome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S-5.8S-25S rDNA site. Both cytological approaches revealed an invariable, heterozygotic karyotype among sweet orange cultivars. Based on these data, the putative hybrid origin of the species is discussed.
Affiliations:
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Schweizer</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14</s1><s2>1030, Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Guerra, M" sort="Guerra, M" uniqKey="Guerra M" first="M." last="Guerra">M. Guerra</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14</s1><s2>1030, Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">00-0166955</idno><date when="2000">2000</date><idno type="stanalyst">PASCAL 00-0166955 INIST</idno><idno type="RBID">Pascal:00-0166955</idno><idno type="wicri:Area/PascalFrancis/Corpus">000990</idno><idno type="wicri:Area/PascalFrancis/Curation">000014</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000967</idno><idno type="wicri:doubleKey">0040-5752:2000:Pedrosa A:cytological:heterozygosity:and</idno><idno type="wicri:Area/Main/Merge">003228</idno><idno type="wicri:Area/Main/Curation">003015</idno><idno type="wicri:Area/Main/Exploration">003015</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cytological heterozygosity and the hybrid origin of sweet orange [Citrus sinensis (L.) Osbeck]</title><author><name sortKey="Pedrosa, A" sort="Pedrosa, A" uniqKey="Pedrosa A" first="A." last="Pedrosa">A. Pedrosa</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14</s1><s2>1030, Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Schweizer, D" sort="Schweizer, D" uniqKey="Schweizer D" first="D." last="Schweizer">D. Schweizer</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14</s1><s2>1030, Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Guerra, M" sort="Guerra, M" uniqKey="Guerra M" first="M." last="Guerra">M. Guerra</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Cytology and Genetics, Institute of Botany, University of Vienna, Rennweg 14</s1><s2>1030, Vienna</s2><s3>AUT</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Theoretical and Applied Genetics</title><title level="j" type="abbreviated">Theor. Appl. Genet.</title><idno type="ISSN">0040-5752</idno><imprint><date when="2000">2000</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Theoretical and Applied Genetics</title><title level="j" type="abbreviated">Theor. Appl. Genet.</title><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome banding</term><term>Citrus fruit</term><term>Citrus sinensis</term><term>Cultivar</term><term>Cytogenetics</term><term>Gene</term><term>Interspecific hybridization</term><term>Ribosomal RNA</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cultivar</term><term>Hybridation interspécifique</term><term>Cytogénétique</term><term>Bande chromosomique</term><term>RNA ribosomique</term><term>Gène</term><term>Citrus sinensis</term><term>Agrume</term><term>Bande chromosomique CMA</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Agrume</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus sinensis chromosomes, although small in size, present a remarkable differentiation of bands with the fluorochromes CMA and DAPI. These bands suggest that some heteromorphisms are fixed in this species. To investigate the extension of these heteromorphisms, ten cultivars of C. sinenesis were analysed with CMA/DAP1 staining and, in some of them, the 18S-5.8S-25S rRNA and 5S rRNA genes were located by in situ hybridization. CMA/DAPI staining showed exactly the same CMA<sup>+</sup>/DAPI<sup>-</sup> banding pattern for all cultivars. In situ hybridization revealed three 18S-5.8S-25S rRNA gene sites, two proximally located on two similar chromosomes and one terminally located on a third non-related chromosome. Two 5S rRNA gene sites were observed in this species, with one located proximal to the telomeric 18S-5.8S-25S rDNA site. Both cytological approaches revealed an invariable, heterozygotic karyotype among sweet orange cultivars. Based on these data, the putative hybrid origin of the species is discussed.</div></front></TEI><affiliations><list><country><li>Autriche</li></country><region><li>Vienne (Autriche)</li></region><settlement><li>Vienne (Autriche)</li></settlement></list><tree><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Pedrosa, A" sort="Pedrosa, A" uniqKey="Pedrosa A" first="A." last="Pedrosa">A. Pedrosa</name></region><name sortKey="Guerra, M" sort="Guerra, M" uniqKey="Guerra M" first="M." last="Guerra">M. Guerra</name><name sortKey="Schweizer, D" sort="Schweizer, D" uniqKey="Schweizer D" first="D." last="Schweizer">D. Schweizer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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